Three-dimensional microfluidic chip for the extraction of mitochondrial DNA
Identifieur interne : 002672 ( Main/Exploration ); précédent : 002671; suivant : 002673Three-dimensional microfluidic chip for the extraction of mitochondrial DNA
Auteurs : Chen-Min Chang [Taïwan] ; Li-Fang Chiou [Taïwan] ; Chun-Che Lin [Taïwan] ; Dar-Bin Shieh [Taïwan] ; Gwo-Bin Lee [Taïwan]Source :
- Microfluidics and Nanofluidics [ 1613-4982 ] ; 2010-08-01.
English descriptors
- KwdEn :
Abstract
Abstract: This study reports an integrated microfluidic chip manufactured by micro-electro-mechanical-system (MEMS) technology used for the automatic extraction of mitochondrial deoxyribonucleic acid (mtDNA). Mitochondria is a key organelle associated with aging, degenerative diseases, and cancers. An alteration in mtDNA may be induced by oxidative stress in tissues and cells and may be used as an indicator for diseases associated with aging. Hence, there is a great need to establish a quantitative and qualitative measurement method to evaluate dysfunction in mitochondria. Traditional processes for the measurement of alterations in mtDNA involves micro-cloning, direct sequencing, real-time polymerase chain reaction (PCR) processing, and microarray detection; all of which are time-consuming and labor-intensive processes. Especially, the extraction of mtDNA from cell samples that require precision and a lengthy process. This microfluidic chip integrates micropumps, a micromixer and a micro temperature module in a three-dimensional structure to automate the entire process of the extraction of the mtDNA. Compared with its conventional counterparts, the advantages of this microchip include less sample and reagent consumption, higher extraction performance, a shorter analysis time, and automation. The developed microsystem is promising for the extraction of mtDNA and can be applied in further mitochondrial studies.
Url:
DOI: 10.1007/s10404-010-0565-8
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000657
- to stream Istex, to step Curation: 000657
- to stream Istex, to step Checkpoint: 000543
- to stream Main, to step Merge: 002697
- to stream Main, to step Curation: 002672
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Three-dimensional microfluidic chip for the extraction of mitochondrial DNA</title><author><name sortKey="Chang, Chen Min" sort="Chang, Chen Min" uniqKey="Chang C" first="Chen-Min" last="Chang">Chen-Min Chang</name></author><author><name sortKey="Chiou, Li Fang" sort="Chiou, Li Fang" uniqKey="Chiou L" first="Li-Fang" last="Chiou">Li-Fang Chiou</name></author><author><name sortKey="Lin, Chun Che" sort="Lin, Chun Che" uniqKey="Lin C" first="Chun-Che" last="Lin">Chun-Che Lin</name></author><author><name sortKey="Shieh, Dar Bin" sort="Shieh, Dar Bin" uniqKey="Shieh D" first="Dar-Bin" last="Shieh">Dar-Bin Shieh</name></author><author><name sortKey="Lee, Gwo Bin" sort="Lee, Gwo Bin" uniqKey="Lee G" first="Gwo-Bin" last="Lee">Gwo-Bin Lee</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:74466BEE6C1966D9BB887F9811A69AE73C419045</idno><date when="2010" year="2010">2010</date><idno type="doi">10.1007/s10404-010-0565-8</idno><idno type="url">https://api.istex.fr/ark:/67375/VQC-5HWS8TBP-B/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000657</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000657</idno><idno type="wicri:Area/Istex/Curation">000657</idno><idno type="wicri:Area/Istex/Checkpoint">000543</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000543</idno><idno type="wicri:doubleKey">1613-4982:2010:Chang C:three:dimensional:microfluidic</idno><idno type="wicri:Area/Main/Merge">002697</idno><idno type="wicri:Area/Main/Curation">002672</idno><idno type="wicri:Area/Main/Exploration">002672</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Three-dimensional microfluidic chip for the extraction of mitochondrial DNA</title><author><name sortKey="Chang, Chen Min" sort="Chang, Chen Min" uniqKey="Chang C" first="Chen-Min" last="Chang">Chen-Min Chang</name><affiliation wicri:level="1"><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Engineering Science, National Cheng Kung University, 1 University Rd, 70101, Tainan</wicri:regionArea><wicri:noRegion>Tainan</wicri:noRegion></affiliation></author><author><name sortKey="Chiou, Li Fang" sort="Chiou, Li Fang" uniqKey="Chiou L" first="Li-Fang" last="Chiou">Li-Fang Chiou</name><affiliation wicri:level="1"><country xml:lang="fr">Taïwan</country><wicri:regionArea>Institute of Oral Medicine, National Cheng Kung University, 1 University Rd, 70101, Tainan</wicri:regionArea><wicri:noRegion>Tainan</wicri:noRegion></affiliation></author><author><name sortKey="Lin, Chun Che" sort="Lin, Chun Che" uniqKey="Lin C" first="Chun-Che" last="Lin">Chun-Che Lin</name><affiliation wicri:level="1"><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Engineering Science, National Cheng Kung University, 1 University Rd, 70101, Tainan</wicri:regionArea><wicri:noRegion>Tainan</wicri:noRegion></affiliation></author><author><name sortKey="Shieh, Dar Bin" sort="Shieh, Dar Bin" uniqKey="Shieh D" first="Dar-Bin" last="Shieh">Dar-Bin Shieh</name><affiliation wicri:level="1"><country xml:lang="fr">Taïwan</country><wicri:regionArea>Institute of Oral Medicine and Department of Stomatology, Advanced Optoelectronic Technology Center, Center for Micro/Nano Science and Technology, National Cheng Kung University, 1 University Rd, 70101, Tainan</wicri:regionArea><wicri:noRegion>Tainan</wicri:noRegion></affiliation><affiliation wicri:level="1"><country wicri:rule="url">Taïwan</country></affiliation></author><author><name sortKey="Lee, Gwo Bin" sort="Lee, Gwo Bin" uniqKey="Lee G" first="Gwo-Bin" last="Lee">Gwo-Bin Lee</name><affiliation wicri:level="1"><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Engineering Science, National Cheng Kung University, 1 University Rd, 70101, Tainan</wicri:regionArea><wicri:noRegion>Tainan</wicri:noRegion></affiliation><affiliation wicri:level="1"><country wicri:rule="url">Taïwan</country></affiliation></author></analytic><monogr></monogr><series><title level="j">Microfluidics and Nanofluidics</title><title level="j" type="abbrev">Microfluid Nanofluid</title><idno type="ISSN">1613-4982</idno><idno type="eISSN">1613-4990</idno><imprint><publisher>Springer-Verlag</publisher><pubPlace>Berlin/Heidelberg</pubPlace><date type="published" when="2010-08-01">2010-08-01</date><biblScope unit="volume">9</biblScope><biblScope unit="issue">2-3</biblScope><biblScope unit="page" from="489">489</biblScope><biblScope unit="page" to="498">498</biblScope></imprint><idno type="ISSN">1613-4982</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1613-4982</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>High efficiency</term><term>Microchip</term><term>Microfluidics</term><term>Mitochondrial DNA</term><term>Nanofluidics</term><term>Rapid extraction</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: This study reports an integrated microfluidic chip manufactured by micro-electro-mechanical-system (MEMS) technology used for the automatic extraction of mitochondrial deoxyribonucleic acid (mtDNA). Mitochondria is a key organelle associated with aging, degenerative diseases, and cancers. An alteration in mtDNA may be induced by oxidative stress in tissues and cells and may be used as an indicator for diseases associated with aging. Hence, there is a great need to establish a quantitative and qualitative measurement method to evaluate dysfunction in mitochondria. Traditional processes for the measurement of alterations in mtDNA involves micro-cloning, direct sequencing, real-time polymerase chain reaction (PCR) processing, and microarray detection; all of which are time-consuming and labor-intensive processes. Especially, the extraction of mtDNA from cell samples that require precision and a lengthy process. This microfluidic chip integrates micropumps, a micromixer and a micro temperature module in a three-dimensional structure to automate the entire process of the extraction of the mtDNA. Compared with its conventional counterparts, the advantages of this microchip include less sample and reagent consumption, higher extraction performance, a shorter analysis time, and automation. The developed microsystem is promising for the extraction of mtDNA and can be applied in further mitochondrial studies.</div></front></TEI><affiliations><list><country><li>Taïwan</li></country></list><tree><country name="Taïwan"><noRegion><name sortKey="Chang, Chen Min" sort="Chang, Chen Min" uniqKey="Chang C" first="Chen-Min" last="Chang">Chen-Min Chang</name></noRegion><name sortKey="Chiou, Li Fang" sort="Chiou, Li Fang" uniqKey="Chiou L" first="Li-Fang" last="Chiou">Li-Fang Chiou</name><name sortKey="Lee, Gwo Bin" sort="Lee, Gwo Bin" uniqKey="Lee G" first="Gwo-Bin" last="Lee">Gwo-Bin Lee</name><name sortKey="Lee, Gwo Bin" sort="Lee, Gwo Bin" uniqKey="Lee G" first="Gwo-Bin" last="Lee">Gwo-Bin Lee</name><name sortKey="Lin, Chun Che" sort="Lin, Chun Che" uniqKey="Lin C" first="Chun-Che" last="Lin">Chun-Che Lin</name><name sortKey="Shieh, Dar Bin" sort="Shieh, Dar Bin" uniqKey="Shieh D" first="Dar-Bin" last="Shieh">Dar-Bin Shieh</name><name sortKey="Shieh, Dar Bin" sort="Shieh, Dar Bin" uniqKey="Shieh D" first="Dar-Bin" last="Shieh">Dar-Bin Shieh</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002672 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002672 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:74466BEE6C1966D9BB887F9811A69AE73C419045
|texte= Three-dimensional microfluidic chip for the extraction of mitochondrial DNA
}}
| This area was generated with Dilib version V0.6.33. |  |